HKBU, China

Prof. Cai graduated from Xiamen University, China in 1982 with a B.Sc. degree and University of Marburg , Germany in 1990 with a Ph.D. degree. He worked in University of Nebraska, USA as a post-doc from 1991-93 and ...

Research Assistant Professor from 1994-96. He was an Associate Professor in the Department of Chemistry, Hong Baptist University from 2001-05; Professor from 2005-10; and Chair Professor since 2010. Prof. Cai served as Department Chair from 2007-11. Currently he is Director of Dioxin Analysis Laboratory, and Director of State Key Laboratory of Environmental and Biological Analysis. The major research interest of Prof. Cai is method development and applications of mass spectrometry for trace analysis in complex systems such as environment, food and biological matrices, and for research focusing on pollutants associated diseases and human health.

 

Abstract


Mass Spectrometry-Based Metabolomics and Imaging Analysis in Research of Environmental Toxicology

Zongwei Cai1,*, Chao Zhao1

1 State Key Laboratory of Environmental and Biological analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong SAR, China

The effects of bisphenol S (BPS), an alternative to BPA, on breast tumor proliferation and deterioration were investigated. Potential molecular mechanisms via molecular biology and mass spectrometry-based lipidomics, proteomics and matrix-assisted laser desorption/ionization-MS imaging (MALDI-MSI) in human breast cancer xenograft model were studied. Oral exposure to mouse models with breast tumor xenograft at low (BPS-10: 10 μg/kg body weight/day) and high (BPS-100: 100 μg) levels were found to significantly promote proliferation of breast tumor. Our results demonstrated that BPS exposure remarkable perturbed abundances of 91 potential markers that mainly involved in five metabolic pathways. We elucidated the lipids spatial heterogeneity by using morphological analysis, probabilistic latent semantic analysis and co-registered multimodal three-dimensional (3D)-MSI. The obtained results suggested that BPS significantly induced tumor proliferation and heterogeneous by the variation of spatial distribution of proteins that were related to chromosomal stability and cell proliferation, lipids signaling, reprogramming of DNA methylation and hypoxic tumor microenvironment in central necrotic domains. BPS exposure caused the tumor deterioration by the variation of spatial distribution of proteins that were associated with the stability of nucleic acid structure, as well as structural lipids in peripheral neoplastic domains of breast tumor. Similar to BPA, BPS has growth-promoting effects on breast tumor in the mice model, which might increase the susceptibility of breast cancer. Furthermore, our results demonstrated the great potential of MALDI-MSI for morphology-driven toxicological evaluation induced by environmental pollutants.


[1] S. A. Morad, M. C. Cabot, Nat. Rev. Cancer, 2013, 13, 51-65.

[2] J. N. Wang, S. L. Qiu, S. M. Chen, C. Q. Xiong, et al. Anal. Chem2015, 87, pp 422-430.

[3] C. Zhao, Z. Tang, J. C. Yan, J. Fang, H. L. Wang, Z. W. Cai, Sci. Total Environ. 2017, 592, 357-365.

[4] Chao Zhao, Peisi Xie, Ting Yong, Hailin Wang, Arthur Chi Kong Chung, Zongwei Cai. Anal Chem. 2018 Feb 16. doi: 10.1021/acs.analchem.7b04540.